DOT Drug Testing + DNA Masterclass
Hear From Our Graduates
Ranked #1 Training Center in McDonough, Georgia
Recognized by BusinessRate • 2026
Lifeline Health Institute is based in Atlanta, Georgia, with scheduled in-person training locations in select cities nationwide. Our DOT training meets federal qualification requirements under 49 CFR Part 40.33 and is led by experienced, qualified instructors.
Qualified & Accredited DOT and Non-DOT collector Training Program
Get trained. Get certified. Build your business.
Get hands-on training, earn 10 industry certifications, and learn how to offer in-demand drug testing and mobile specimen collection services.
Want to take it further? Add professional laboratory account setup to your Masterclass or enroll in our exclusive Train-the-Trainer Program to teach your own employees and students.
Training Locations
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Atlanta, GA
Lifeline Health Institute
January 10, 2026
Registration Deaadline: Jan. 1st
Seats Remaining: 8
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Charlotte, NC
Staybridge Suites - Meeting Room
November 29, 2026
Registration Dealdline: Nov. 20th
Seats Remaining: 2
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Dallas, TX
Staybridge Suites - Meeting Room
December 13, 2026
Registration Dealdline: Dec. 4th
Seats Remaining: 14
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Detroit, MI
Staybridge Suites - Meeting Room
January 3, 2026
Regisration Deadline: Dec. 25th
Seats Remaining: 10
Not ready for the full masterclass? Start with our free online DOT urine collection course. 5 required mock collections are available separately for $97.
EARN 10 CERTIFICATIONS.
More Service Can Mean More Opportunities for Contracts!
Turn one day of hands-on training into multiple ways to generate revenue.
$997
Afterpay Payment Plan Availavle at Checkout
Our Masterclass is designed for aspiring entrepreneurs, business owners, healthcare professionals, and individuals ready to enter the drug testing and specimen collection industry.
No medical experience.
This isn't just a certification class. You'll learn how to perform the collections and receive step-by-step business guidance, vendor resources, account setup information, and tools to help you turn your certifications into a working mobile testing business.
YOUR 10-CERTIFICATION TRAINING
DOT Testing
✔ DOT Urine Drug Testing Certification
✔ DOT Oral Fluid Testing Certification
✔ Breath Alcohol Technician (BAT) Certification
✔ Screening Test Technician (STT) Certification
Non-DOT & Specialty Testing
✔ Non-DOT Urine Drug Testing Certification
✔ Non-DOT Oral Fluid Testing Certification
✔ Hair Drug Testing Certification
✔ Fingernail Drug Testing Certification
✔ DNA Collection Certification
✔ Ink Fingerprinting Training
Hands-on mock collections are included. You'll practice the collection process in class so you're not leaving with certifications alone, you'll understand how to actually perform the services.
One Training. Multiple Ways to Build Your Business.
You're not leaving with just one service to offer. Your training prepares you to provide multiple testing and collection services to individuals, employers, organizations, and other businesses.
From mobile and on-site collections to workplace testing, DNA collections, breath alcohol testing, fingerprinting and more, your certifications give you multiple ways to serve clients and grow your business.
But we don't stop at teaching you how to perform the collections.
We Show You How to Start Building the Business.
You'll receive guidance, resources, and industry information to help you begin putting your new skills to work, including:
✔ Industry Vendors & Resources — access to vendor information so you know where to obtain supplies, products, and services for your business.
✔ Gender Reveal Testing — vendor resources for adding gender reveal collection services to your service menu.
✔ Government Contracting — an introduction to finding and pursuing government contracting opportunities for your business.
✔ Business Startup & Operations — guidance for structuring your services and getting your mobile testing business up and running.
✔ Growing Your Service Menu — learn how additional testing and collection services can help you expand beyond one source of business and create more opportunities to serve clients.
Whether you want to start a mobile testing business, add services to an existing company, or create an additional stream of revenue, you'll leave with more than certifications—you'll have resources to help you determine your next steps.
More certifications. More services. More ways to build your business.
YOU'RE NOT LEFT TO FIGURE IT OUT ALONE
After class, stay connected through the DOT Collector Network, our Facebook community for collectors and mobile testing professionals.
Ask questions, connect with other professionals, access resources, learn about new service opportunities, receive industry updates, and continue learning ways to grow and scale your business.
10 Certifications. Hands-On Collections. Business Setup. Vendor Resources. Finger-Stick Lab Access. Ongoing Community.
Want Us to Set Up Your Lab Account?
Get Certified. Get Your Lab Account Established. Start Building Your Business.
Completing your training is an important first step. Establishing a professional laboratory account is another important step toward operating your own drug testing business.
Rather than navigating the laboratory setup process on your own, let Lifeline Health Institute handle it for you.
Our Masterclass + Lab Account Setup Package includes everything in the 10-Certification Masterclass, PLUS professional account setup assistance with one major laboratory.
What's Included?
✔ All 10 Certifications
✔ Hands-On Training & Mock Collections
✔ Mobile Lab Business Training
✔ Industry Vendors & Resources
✔ Laboratory Account Setup with One Major Laboratory
Available Laboratories:
Quest Diagnostics | Labcorp | Clinical Reference Laboratory (CRL)
Laboratory selection and account activation are subject to the laboratory's eligibility, documentation, and approval requirements.
Masterclass + Lab Account Setup
$1,397
Focus on learning the skills and building your business while we help handle the laboratory account setup process.
Afterpay Available at Checkout.
Want to Go From Collector to Trainer?
Learn the Skills. Build Your Business. Teach Others.
For students who want to take their business even further, Lifeline Health Institute offers an exclusive Train-the-Trainer Program as an add-on to our 10-Certification Masterclass.
Learn how to train your own employees and offer drug testing and specimen collection training through your business.
Whether you're building an internal team or want to offer classes to other individuals entering the industry, this program prepares you to deliver training within the scope of your instructor qualification and applicable regulatory requirements.
Train-the-Trainer Instruction Includes:
✔ DOT Urine Collection
✔ DOT Oral Fluid Collection
✔ Breath Alcohol Testing
✔ Non-DOT Urine & Oral Fluid Collection
✔ Screening Test Technician Procedures
✔ Hair & Fingernail Collection
Your Train-the-Trainer Package Also Includes:
Complete 10-Certification Masterclass
Attend the full hands-on Masterclass and complete your collector training.
Professional Laboratory Account Setup
Receive account setup assistance with one major laboratory: Quest Diagnostics, Labcorp, or CRL, subject to laboratory approval.
Live Virtual Instructor Training
Participate in live virtual Train-the-Trainer instruction designed to help you prepare to train employees and students.
Training & Business Guidance
Learn how to structure your training services, prepare to instruct students, and incorporate training into your existing or future business.
An Exclusive Opportunity for Our Masterclass Students
The Train-the-Trainer Program is available only to Lifeline Health Institute Masterclass students and must be selected at the time of Masterclass enrollment.
It is not available as a standalone program or for enrollment at a later date.
Masterclass + Lab Account +Train-the-Trainer Program
Masterclass + Train-the -Trainer: $2597
Your live virtual Train-the-Trainer training will be scheduled after your lab account is setup.
Afterpay Available at checkout
Ready to Get Started?
Qualified & Accredited DOT and Non-DOT collector Training Program
Frequently Asked Questions
Does your DOT training meet federal qualification requirements?
Yes. Lifeline Health Institute's DOT training is designed to meet the applicable training and qualification requirements under 49 CFR Part 40.
Our program provides instruction in federally regulated drug and alcohol testing procedures, including the knowledge and practical skills needed to perform DOT collections. Students must successfully complete the applicable proficiency demonstrations and other qualification requirements.
Do I need medical or drug testing experience to attend the Masterclass?
No. Prior medical or drug testing experience is not required.
Our Masterclass is designed for beginners, aspiring entrepreneurs, healthcare professionals, and individuals interested in starting or expanding a drug testing and mobile lab business.
We provide step-by-step instruction, practical demonstrations, and hands-on training.
What certifications are included in the Masterclass?
Our 10-Certification Masterclass includes:
DOT Urine Specimen Collection
DOT Oral Fluid Collection
DOT Breath Alcohol Testing (BAT)
Non-DOT Urine Collection
Non-DOT Oral Fluid Collection
Non-DOT Screening Test Technician (STT)
Hair Specimen Collection
Fingernail Collection
DNA Specimen Collection
Ink Fingerprinting
The program also covers business operations, pricing, marketing, employer accounts, mobile testing services, and strategies for establishing your own drug testing business.
Are mock collections included in the training?
Yes. All required mock collections and proficiency demonstrations included in your training program are covered by your tuition.
Students receive hands-on instruction and practical training opportunities to demonstrate their collection skills.
There are no additional Lifeline Health Institute fees for the mock collections included in the program.
How long is the Masterclass, and what is included?
Our in-person Masterclass is a one-day intensive training program held from 10:00 AM to 4:00 PM.
The program includes classroom instruction, hands-on demonstrations, practical training, business education, training materials, and industry resources.
Continental breakfast and lunch are also provided for in-person attendees.
What is the cost of the Masterclass?
We offer three enrollment options:
Option 1: Masterclass Only — $997
Includes our 10-certification training program, hands-on instruction, mock collections, training materials, and business education.
Option 2: Masterclass + Lab Account Setup — $1,397
Includes everything in the Masterclass, plus assistance establishing an account with one participating major laboratory, such as Quest Diagnostics, Labcorp, or Clinical Reference Laboratory (CRL).
Option 3: Masterclass + Train-the-Trainer + Lab Account Setup — $2,597
Includes the complete Masterclass, Train-the-Trainer Program, and Lab Account Setup assistance.
All packages must be paid in full at registration. Afterpay is available at checkout for eligible customers who prefer payment arrangements.
Important: Train-the-Trainer and Lab Account Setup are only available at the time of initial enrollment and cannot be added later.
Do you offer payment plans?
Yes. We offer Afterpay at checkout for eligible students who prefer to divide their purchase into payments.
What is the Train-the-Trainer Program?
Our Train-the-Trainer Program is an advanced training opportunity exclusively available to Masterclass students who want to go beyond performing collections and learn how to train others.
The program prepares you to train employees and offer drug testing and specimen collection training through your own business.
What Train-the-Trainer programs are included?
Train-the-Trainer instruction covers:
DOT Urine Collection
DOT Oral Fluid Collection
DOT Breath Alcohol Testing (BAT)
Non-DOT Urine Collection
Non-DOT Oral Fluid Collection
Hair Collection
Fingernail Collection
The package also includes Lab and MRO Account Setup assistance to help establish the infrastructure for your testing business.
Can I offer my own drug testing classes after completing Train-the-Trainer?
Yes. Our Train-the-Trainer Program is designed to prepare students to train employees or offer drug testing and specimen collection classes through their own businesses.
You will learn how to transition from performing collections to instructing others while following applicable training and qualification requirements.
When will I take my Train-the-Trainer class?
Your Train-the-Trainer class is scheduled after you complete your live, in-person Masterclass.
Train-the-Trainer instruction is conducted live via Zoom, allowing you to complete your instructor training without returning to one of our training cities.
Can I add Train-the-Trainer after completing the Masterclass?
No. Train-the-Trainer is exclusively available as an add-on at the time of your initial Masterclass enrollment.
It cannot be purchased separately or added after registration, even if you have not yet attended your scheduled Masterclass.
If becoming an instructor is part of your future business plans, you must select the Masterclass + Train-the-Trainer package when registering.
What is Lab Account Setup Assistance?
Lab Account Setup Assistance helps students establish a laboratory account for their drug testing business.
We assist with the account setup process for one participating major laboratory, such as Labcorp, Quest Diagnostics, or Clinical Reference Laboratory (CRL).
Account approval and activation are subject to the laboratory's requirements.
Can I purchase Lab Account Setup after registering?
No. Lab Account Setup is available exclusively as an add-on during initial Masterclass enrollment.
Students interested in this service must select either the Masterclass + Lab Account Setup package or the Masterclass + Train-the-Trainer + Lab Account Setup package when registering.
This service cannot be added or purchased separately after enrollment.
Are payments refundable or transferable?
We offer a 72-hour cancellation and refund period beginning at the time of registration.
Students who submit a cancellation request within 72 hours of registering are eligible for a full refund.
After the initial 72-hour period, all enrollment and program payments become non-refundable and non-transferable.
This policy is in place because our administrative and student onboarding processes begin shortly after enrollment. Students are entered into our training systems, enrollment records are established, and preparations begin for training materials, supplies, certifications, and program resources.
Students who cancel and receive a refund during the 72-hour cancellation period will not be eligible for readmission into future Lifeline Health Institute classes.
Please carefully review your training date, enrollment package, and program policies before registering.
Can I transfer my registration to another class date or another person?
No. Registrations are non-transferable after the 72-hour cancellation period.
Each registration is associated with an individual student and a specific training date. Please confirm your availability before completing enrollment.
Why should I consider Train-the-Trainer now?
If your long-term goals include training employees, building a team, or offering your own drug testing classes, Train-the-Trainer provides an opportunity to expand beyond performing collections yourself.
Instead of building a business solely around the services you personally perform, you can develop the skills to teach those services to others.
Because Train-the-Trainer is only available during initial enrollment, we encourage students to consider their long-term business goals before selecting their package.
How do I register for the Masterclass?
Registration is completed online through our website.
Select your preferred training location and date, review the three enrollment packages, and complete checkout.
Full payment is required to secure your seat. If you prefer payment arrangements, Afterpay is available at checkout for eligible purchases.
Once your registration is successfully processed, you will receive enrollment confirmation and additional information regarding your scheduled training.
Qualified & Accredited DOT and Non-DOT collector Training Program